Bioinformatics and genomics for public health and clinical microbiology
prokka. :zap: :aquarius: Rapid prokaryotic genome annotation
993snippy. :scissors: :zap: Rapid haploid variant calling and core genome alignment
594abricate. :mag_right: :pill: Mass screening of contigs for antimicrobial and virulence genes
502barrnap. :microscope: :leo: Microbial RNA annotation
306mlst. :id: Scan contig files against PubMLST typing schemes
295shovill. ⚡♠️ Assemble bacterial isolate genomes from Illumina paired-end reads
261snp-dists. Pairwise SNP distance matrix from a FASTA sequence alignment
156any2fasta. Convert various sequence formats to FASTA
148nullarbor. :floppy_disk: :page_with_curl: "Reads to report" for public health and clinical microbiology
144samclip. Filter SAM file for soft and hard clipped alignments
53VelvetOptimiser. :chart_with_upwards_trend: Automatically optimise three of Velvet's assembly parameters.
50PEAR. Pair-End AssembeR
35phastaf. Identify phage regions in bacterial genomes for masking purposes
33cgmlst-dists. 🐻⇔🐨 Calculate distance matrix from ChewBBACA cgMLST allele call tables
29seeka. Get microbial sequence data easier and faster
29berokka. 🍊 💫 Trim, circularise and orient long read bacterial genome assemblies
28homebrew-bioinformatics-linux. :beer: :penguin: Homebrew formulae for bioinformatics software only available for Linux
27sixess. 🔬🐛 Rapid 16s rRNA identification from isolate FASTQ files
25ekidna. Assembly based core genome SNP alignments for bacteria
25scripts. Miscellaneous Perl scripts from 20 years in microbial bioinformatics
18scapper. Whole genome core alignments from multiple draft genomes
13legsta. 🍗⭐ In silico Legionella pneumophila Sequence Based Typing
12kounta. 🧮 🔢 Generate multi-sample k-mer count matrix from WGS
11mokka. Annotate your metagenome assemblies
11tseemann.github.io. Torsten Seemann's Home Page
9snasm. Assembly based core SNP alignments
8noary. 🍣 🦐 A lightweight nucleotide bacterial ortholog clustering tool
7wombac. :bangbang: Rapid core genome SNP alignments from multiple bacterial genomes
7trencha. Normalize VCF depth for Illumina GC bias
7klosham. Find closest aligned sequences to a query sequnece
6kopynumba. Identify copy number variation in bacterial Illumina sequences
6spekki. Species prediction from NGS reads
5injecta. Insert genes into genomes to aid synthetic test data generation
5polyfix. 🔪⛓️ Repair nanopore assemblies using related genome(s)
5skrofula. Yet another M.tuberculosis typing and resistance tool, but for the impatient (not in-patient)
5fasterqc. A non-Java alternative to the classic FastQC tool
5varion.
5ragnarokka. Annotate and correct erro-prone ONT genomes
4bowkaster. cgMLST from FASTQ reads
4kurra. Fast whole genome phylogeny
4polisha. Fix small assembly errors using Illumina reads
4babykraken. 👶🦑 Very small Kraken2 database for bundling with pipelines
4dehomopolymerate. Collapse sequence homopolymers to a single character
4heterik. Estimate heterozygosity or mixture level of a bacterial WGS sample
3perl-biotool. 🐫 🐪 Small pure Perl5 libraries for writing command line bioinformatics tools
3anthrakks. Distinguish Bacillus cereus and biovar anthracis (anthrax)
3bioinfo-scripts. Collection of bioinformatics utility scripts, mostly written in Bioperl
3gbk2bcfgff. Convert Genbank to GFF compatible with "bcftools csq"
2mini-outbreak. Small WGS dataset for testing bacterial outbreak analysis pipelines
2snippa. Experimental modular bacterial SNP calling pipeline
2easy-web-blast.
2kroucha. Mock repository for Sanger publications citing Croucher et al
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